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We selected a C-60 suspension that has previously been shown to provoke cell membrane destabilisation in vivo.
In the intrinsic pathway (route 2), diverse stimuli that provoke cell stress or damage typically activate one or more members of the BH3-only protein family.
Here we demonstrated that CRISPR-Cas9 system can effectively trigger programmable double strand break (DSB) at the chromosome of PCC 7942 and provoke cell death.
This study constitutes the first report of a PTP-targeted strategy able to selectively open the PTP and consecutively provoke cell death in some cells whilst sparing others.
The proinflammatory cytokine TNFα fails to provoke cell death in isolated hepatocytes but has been implicated in hepatocyte apoptosis during liver diseases associated with chronic inflammation.
We found earlier that glutathione reductase (GR), having higher inherent NADPH oxidase activity in vitro than SecTRAPs, could not provoke cell death [37].
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Based on gene ontology annotations, we have further discriminated between two classes of kinases that, when suppressed, result in alterations of the mitotic index and provoke cell-cycle arrest.
It forms pores in the cell and mitochondrial membrane of neutrophils and macrophages and thus provokes cell lysis and apoptosis with subsequent liberation of inflammatory mediators [4, 7].
A single type of mechanical stimuli in vitro often provokes cell's sensing and responding to external forces via multiple molecular events (Fig. 1A).
However, if degradation of Pfkfb3 is inhibited, glycolysis is stimulated and oxidative stress results [56]; in neurones this provokes cell death [57].
As expected, the ingestion of concentrated sera provoked cell death.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com