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We wish to thank Andrew Reynolds and Hodivala-Dilke for assistance with the aortic ring assay, Grégory Bieler and Camille Despland for producing P21 silenced HUVEC and Eveline Faes for providing the protocol of HUVEC spheroid assay.
We thank Prof. Luigi Naldini for providing the plasmids for lentiviral production; Dr. Nicole Deglon for providing the protocol for in vivo lentiviral injection; and Prof. Stefano Vicini for providing the coculture protocol and helpful advice.
We are grateful to Maria Carvalho for providing the protocol for chemical sulfation of sterol lipids.
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This provides the protocol user with the knowledge of exactly what he/she needs to have in place before commencing.
Here, we describe the pathway and provide the protocols and supporting tools necessary for wider use.
Carl-Johan Carl-Johan Rubinedged for provising the chromosome wacknowledgedcol.
We appreciate Dr. Paxton Payton for providing the RNA isolation protocol.
C.H.H. and C.A.K. provided the modified protocol for global mRNA amplification.
This study provides the simplest protocol to prevent proline artifacts in SILAC labeling experiments with arginine.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com