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We thank Professors Izumi Kumagai and Mitsuo Umetsu (Tohoku University) for providing sequence data for the scFv-encoding fragment.
In addition to providing sequence data that can be used for benchmarking analytical techniques for metagenomics, it is our hope that this type of simulation can help aid model development for future in silico simulations.
Consequently, unless the sheared DNA population has a wide size distribution, it will contain very few fragments capable of providing sequence that overlaps with the telomeric reads.
Our assembly integrates high-throughput short reads, long reads, and a genome map providing sequence for greater than 90% of the predicted genome size.
A total of 563,163 contigs resulted in BLAST hits to non-protein coding regions of the genome, providing sequence information that is unavailable from EST sequencing experiments.
We thank Kim Kyung and Robert Fulton (WUGSC) for providing sequence of the small gap in our dog BAC [GenBank: AC090445].
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An alternate scenario can also be envisioned wherein the extension may have evolved from the protruding domain, providing sequence-continuous domain definitions.
Regions spanning a few hundred base pairs either side of the apparent trans-splicing sites in both genes were amplified from genomic DNA and cloned, providing sequence-verified DNA templates.
In addition, Brassica species share an ancestor with Arabidopsis, implying a similar basic genome, and thereby providing sequence-level colinearity between the two genera, particularly in euchromatic regions [ 7- 9].
We thank Roche, Illumina and Pacific Biosciences corporations for providing sequencing and computational resources.
(BTSS) for samples collection, The GenePool for providing sequencing service, UKM Medical Molecular Biology Institute (UMBI) in providing facility for fluorescent microplate reader.
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