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This zone is weak and weaker than the ligaments and provides a cleavage pathway for the fracture.
For instance, the unique insertion, IN4, provides a cleavage site for Asp-N endopeptidase metalloprotease, which has been detected in the skin mucosa of salmonids [ 15].
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We highlight the importance of both carrier and cargo labelling, possibly with a pair of fluorophores able to provide a cleavage-responsive signal such as FRET, as tools to achieve a better understanding of our hybrids.
LipoP is an HMM-based predictor of lipoprotein signal peptides in Gram-negative bacteria, while SignalP combines several NNs with HMMs to detect signal peptides in both bacteria and eukaryotes and also provides a prediction of cleavage sites.
These strategies are carried out by combining an AMP with a carrier protein through a linker that provides a site recognition for cleavage of the AMP from the fusion protein.
The proteolytic GFAP fragment produced on caspase cleavage provides a complementary mechanism that could potentially alter GFAP filament organization and filament properties in astrocytes.
This cleavage provides a signal for the polyadenylation of the resulting fragments involving the recruitment of poly(A) polymerase I (PAP I) to the fragments, most likely as a result of its interaction with the degradosome.
This model provides a mechanism by which cleavage-type crack growth could proceed concomitantly with significant plastic dissipation.
One of the advantages of ECD over the thermal methods is that it provides a more uniform pattern of cleavages along the backbone, with the only exception being cleavage of the N-terminal to proline(28) and thus leads to greater peptide sequence coverage.
The reductive cleavage reaction provides a convenient method to access novel 2-aminonicotine and pyrido[2,3-b]azepine derivatives.
In the presence of D30N/N88D mutations in the protease, the coevolutionary mutation LP1′F at the p1-p6 cleavage site provides a better fit within the dynamic substrate envelope.
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