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The sections were either stained with haematoxylin and eosin (DAKO, Glostrup, Denmark) following the providers protocol or blocked with 1% BSA/TBS prior to immunostaining.
Nucleic acid extraction was performed as reported elsewhere [ 21] using ultracentrifugation and caesium chloride method followed by the phenol/chloroform purification or using the DNA extraction kit from Qiagen, Germany, using the providers protocol.
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Cells were cultured according to providers' protocols.
All hESC lines were grown under similar conditions, with variations dictated by the providers' protocols.
The lines were grown on feeders, or feeder-free on fibronectin, using bovine, human, or artificial serum according to the providers' protocols (see Methods).
Another study in Ethiopia found that increase in workload during integration may also be due to expanded client-provider protocol that increases session times per contact with each client [ 8].
The expression of GM-CSF, IFN- γ, IL-1 α, IL-2, IL-4, IL-5, IL-6, IL-10, IL-17, and TNF- α was measured using the mouse and human Th1/Th2 10plex FlowCytomix Multiplex kit (eBioscience, USA) according to the provider protocol.
Input from community providers during protocol development highlighted potential concerns and helped to address issues of practicality and feasibility.
The company, for example, is working with Red Hat and Codenvy to bring to other tool and language providers the protocol that allows its free Visual Studio Code editor to support more than 100 programming languages already.
Caspase assays were performed according to provider's protocol (Invitrogen).
Primary antibodies were diluted in blocking solution in accordance with provider's protocol.
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