(NIH had not provided reaction as this item went to press).
Square-wave temperature cycles at periods above 5 hr provided reaction rates which, when time-averaged, were closely approximated by quasi-steady state reaction rates.
Similarly, for the T7 RNA polymerase reactions, 2 µl of normalized protein (Ambion) was mixed with 50 nM ds DNA template containing T7 promoter (Figure 2A) in the provided reaction buffer at 37°C for 1 hr.
Fifty microlitres of the supernatant was mixed with 50 μl of the provided reaction buffer.
Briefly, the microparticles and enzyme were suspended in the provided reaction buffer along with 5 mM ATP or TTP.
Following UV exposure, BrdU incorporated strands were digested with Exonuclease III (3U/μl in provided reaction buffer; Promega) at room temperature for 10 minutes.
The final section provides reactions and critique.
Subtext pulls in this kind of supplementary information automatically, providing reactions, commentary, etc. that doesn't completely detract from the actual reading.
Trials may also provide reactions to a specific category or product because of the opportunity that the participants have to experience competing technologies (e.g., conventional gasoline cars) [39].
