We are grateful to the International Rice Research Institute (Los Banos, Philippines) for providing leaf and seed samples.
In the following spring when the trees were cut down to the bud, grafts from the shoots removed from the OHxLBJ seedlings were inserted onto Pyrus calleryana seedling rootstocks to provide leaf material for DNA extraction.
We also thank Kostas Karandemiris for helpful suggestions in EST-database searches, Heiko Schumacher for providing leaf images, Michalis Averof and members of the lab for critical reading of the manuscript.
One of the diploid species, B. rapa, comprises a variety of morphologically diverse cultivated types including Chinese cabbage, misuna, aburana, flowering cabbage, turnip, turnip rape, yellow sarson, tatsoi and komatsuna, and these provide leaf heads, leaves, flowering stems, turnips and seeds, the productive organs for economical consumption [ 3, 4].
In the 19th century, for example, Slotte (2001) estimated that almost nine million trees were pollarded each year in the Swedish forest to provide leaf-hay as winter diet for cattle and sheep.
However, no VC rate provided tomato leaf and flower numbers on a per plant basis that were similar to SFT.
VBW provided the leaf gene data.
The yeast used in both the SSF and SHF experiments was Ethanol Red, kindly provided by Leaf Technologies (Marcq-en-Baroeul Cedex, France).
The collection of samples that provided the leaf material for the study has been labelled the Phytomedicine Tree Database (PMDB), Paraclinical Sciences, Faculty of Veterinary Sciences, University of Pretoria.
As each leaf was cut from the plant, it was immediately placed in a vial with the petiole down and the difference in weight provided the leaf fresh weight (LFW).
