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Stepped care is provided in three steps: 1) guided self-help, 2) cognitive behavioral therapy and 3) antidepressant medication.
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Therefore, the WGS-SNP combination not only eliminates all technical aspects of SNP mapping (PCR, sequencing or restriction mapping of PCR amplicons), but also provides, in one step, a much finer grained resolution.
This work provides improvements in four steps of long distance RT-PCR (L-RT-PCR): (i) preparation of a viral stock, (ii) preparation of template RNA, (iii) reverse transcription and (iv) amplification of the cDNA by LD-PCR.
These oxetane-containing bicycles, readily accessible from simple alkenes in three steps, may provide interesting rigid motifs for medicinal chemistry.
This proof is an extension of the proof provided byRogers and Veraart (2013) and proceeds in three steps.
Starting from compound 1, our route provides 4 in 60 % overall yield in three steps with three chromatographic purifications; in total, 1.9 g of 4 was obtained in the course of this study.
Computation proceeds in three steps.
This happens in three steps.
In three steps.
All analyses were performed in three steps.
Our statistical analyses proceeded in three steps.
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