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Next, screw the track to the top jamb; the necessary wood screws will be provided in the kit.
The purified product was amplified by 18 cycles PCR with SeqAmp DNA polymerase and the Illumina Index Primer set, both provided in the kit.
The AhR Reporter cells (expressing luciferase under AhR promoter) as well as positive control MeBio (AhR ligand) compound were provided in the kit.
Allele calling was achieved by comparison with the allelic ladder provided in the kit, helped by GeneMapper software (version 3.2).
Bovine serum albumin (BSA) provided in the kit was used as the standard curve, and absorbance was read at 560 nm.
Briefly, samples were fixed and incubated with Sirius Red solutions for 30 min at room temperature, and eluted using extraction buffer provided in the kit.
TRAP staining was carried out using a TRAP/alkaline phosphatase (ALP) stain kit (Wako Pure Chemicals Industries Ltd., Osaka, Japan) according to the protocol provided in the kit.
Other supplies not provided in the kit can be purchased separately.
The final calculations were performed by using the formula provided in the kit.
MDA standards provided in the kit were used to quantify the amount of MDA formed.
Thereafter, cells were rinsed once in 1× rinsing buffer provided in the kit.
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