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Additionally, the carbon provided buffer spaces for the volume expansion of the active material.
The proposed scheme uses dynamic adjustment to the provided buffer space.
Cells pellets were washed with PBS once and lysed using the provided buffer.
The last aliquot was incubated at 37°C for 30 minutes with Calf Intestinal Alkaline Phosphatase (Promega) in the provided buffer to remove 5' phosphate groups from transcripts.
The reaction mix consisted of dNTPs (200 µM), AmpliTaq-Gold and provided buffer (Applied Biosystems), MgCl2 (3 mM) and forward/reverse primers (1 picoMolar of each).
Cell lysates were incubated overnight at 4 °C with the array in the provided buffer.
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In layered architectures, each network layer requires overprovisioning of its provided buffers to ensure that all packets can be stored.
Modified DNA was purified using Zymo spin columns along with the provided buffers in the kit.
This undertaking will cost an estimated $8 billion over 20 years, shared by the federal government and Florida, and will also include land acquisition to provide buffer zones between the Everglades and Florida's expanding coastal cities.
The second amendment would require the corps and the district to use Florida's condemnation law when buying the land necessary to restore water flows or provide buffer zones to protect the park from encroaching development.
This strategy provides buffer space, which is constructed by carbon porous continuous conductive framework throughout the entire electrode, to resist local stress and intense volume variation.
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