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In this study, we identified 129 commercial samples using the BOLD "species-level" identification tool to query sample barcodes against the BOLD reference database or using NCBI's BLAST tool, which provided a sequence similarity value greater than 99% in all cases, despite considerable length differences of the query sequences.
Additionally 38 mosquito pools were positive for flavivirus-genus PCR only and 26 provided a sequence due to the specific PCR target (part of the NS5 gene) but not arising from the presence of WNV or USUV.
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Using 2 multiplex PCRs, MLVA of 96 isolates can easily be performed within a single day provided a sequencing facility is readily available.
"The new recommendations are right on target, and provide a sequence of things we can do if the problem gets worse," Dr. Cohen said.
In addition, it provides a sequence of calculations designed to quickly and accurately analyze various parameters of existing bit hydraulics.
Under the same assumptions on the functional, this paper establishes a new critical point theorem which provides a sequence of critical points converging to zero.
The proposed randomized algorithm provides a sequence of candidate solutions converging with probability one to a feasible solution in a finite number of steps.
The tool provides a sequence of low-level operations in a single package that can produce accurate brain segmentations in clinical time.
The latter aims at providing a sequence of updates to follow in order to propagate the modifications on templates to their instances.
One result of this hydrogeologically mediated valley evolution was to provide a sequence of proximal Palaeolithic archaeology over two MIS cycles.
We propose a regularized, pseudo-dynamical recursion scheme that provides a sequence of updates, which are almost insensitive to the regularizing term as well as the time step size used for integrating the pseudo-dynamical form.
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