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This step was complicated by the known propensity of RNA-Seq to provide uneven coverage of transcript termini - presumably due to the dynamics the nebulization step in sample preparation and to issues associated with differential recovery of fragments during sample preparation.
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Candlelight is acceptable in a light room, but in a dark setting, it provides uneven, raw light, made harsh by its sharp contrast with the surrounding gloom.
Unfortunately, we have socialized the benefits of child-rearing more thoroughly than we have socialized the costs, taxing the working-age population to provide benefits to the elderly through Social Security and Medicare but providing uneven and somewhat unpredictable public support to parents.
At the very least we'll need to look at ways to provide less uneven funding to schools and school districts so students' educational chances are less dependent on the circumstances of their birth and more dependent on the choices they actually make in school.
Incorporation of mutant lamin A/C into the nuclear lamina may provide an uneven surface that fails to normally interact with chromatin.
Lower-SES communities usually have limited access to quality housing and live in neighborhoods that do not facilitate outdoor activities and provide an uneven mix of healthy and unhealthy food outlet choices such as few supermarkets but many low-cost restaurants.
The tactics of engaging public opinion are generally avoided by researchers, who regard this as unethical behavior, but harvesting public opinion is core business for politicians and that particular culture clash can provide an uneven playing field, where the research team is disadvantaged.
While Joe Levasseur's lighting, with its sudden, spooky blackouts, provides the uneven work with the most depth, silence is juxtaposed with music from D.J. Filastine's "Dirty Boom".
But in the all-important part of Charlie, Howard Bickle provides an uneven performance.
It must also be taken into consideration that further protection could be provided by uneven codon usage, i.e. in case of multiple codons for one amino acid those codons are preferred that are dissimilar to codons that code for dissimilar amino acids.
The outer diameter of the cup could not be accurately measured with direct measurement since the metal mesh provided an uneven surface, which made automatic measurement with CMM difficult due to the sensitivity of the CMM probe and it would be too time-consuming to do it manually.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com