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These meshes will be overlaid with thermal infra-red data and multi-spectral images data that provide colorimetric information (for chemical composition and photosynthetic functional analysis).
In the final step, the plate was washed and treated with TMB solution (3,3′,5,5′-teramethylbenzidine; American Qualex antibodies, San Clemente, CA, USA) to provide colorimetric quantification.
Recent image-processing solutions, such as TraitMill [ 11] and HTPheno [ 12], provide a more general plant analysis and measure information such as plant height, width, centre of gravity, projected area and bio-volume, and provide colorimetric analysis (e.g. greenness-differences between plants).
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Caspase activation leads to the cleavage of the provided colorimetric substrates (all substrate peptides are conjugated to p-nitroaniline (pNA); caspase 3: DEVD-pNA, caspase 8: IETD-pNA, caspase 9: LEHD-pNA) and can be measured photometrically at 405 nm.
Tetramethylbenzidine (TMB) substrate was added to provide a colorimetric readout and the reaction was stopped by stop solution.
After a second incubation and a simple washing step to remove unbound enzyme-labeled antibody, the bound conjugate was detected through a reaction with 3,3′, 5,5′-tetramethylbenzidine. The reaction was stopped by adding acid to provide a colorimetric endpoint, and was then read using spectrophotometry at 450 nm.
Furthermore, this FOS-SI-LOV methodology provided a colorimetric and fluorescence detection platform for other hazardous species monitoring in water environment based on Beer's law and Stern-Volmer relationship, respectively.
The entire detection is performed under isothermal conditions and provides a colorimetric output, which minimizes the need for instrumentation.
Addition of a secondary HRP-conjugated antibody provides sensitive colorimetric readout quantified by spectrophotometry at 450 nm.
The addition of a secondary horseradish peroxidase (HRP -conjugated antibody and developing solution provided a colorimetric readout tHRP -conjugatedphotometricantibodyntified and450 nm.
Addition of a HRP-conjugated secondary antibody, followed by a developing solution and a stop solution, provides a colorimetric readout which was quantified by measurement of absorbance at 450 nm with a reference wavelength of 655 nm.
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