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In the mining sector, off-gas that is typically exhausted to the environment could be sparged into culture vessels to provide agitation and CO2 as a carbon source for photosynthesis and lipid production (Scott et al.,2010).
In these approaches, a miniature battery-operated electric stirrer was employed to provide agitation of sample solutions instead of the commonly used large size magnetic stirrer powered by alternating current in conventional SBSE process, which could extend the SBSE technique from the conventional off-site analysis to the on-site sampling.
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The extraction was performed by combining wet sediment sample and buffer solution at a 1 1 ratio (v/v) in sterile 50-mL Falcon tube and providing agitation for 4 h at 120 rpm and 4 °C.
Meanwhile, the gas stripping operation also provided agitation and loosened the solid substrate bed, which was not only helpful for the growth of microbes but also improved the mass and heat transfer efficiency through strengthening the solid phase during GS-SSF.
Incubations carried out on board a research vessel will provide some agitation of the bubble but this will not approach the high and constant agitation tested in our experiments.
Containers with square cross sections (10 cm × 10 cm) were used to provide complete agitation.
Rotary drum composting is an efficient and promising technology compared to conventional outdoor technologies such as windrows and static piles, as it provides adequate agitation, aeration and proper compost mixing to produce consistent and uniform end product without any nuisance odor or leachate-related problems (Kalamdhad et al. 2009; Rodríguez et al. 2012).
The impeller must provide sufficiently rapid agitation to disperse all compounds and achieve an effectively homogeneous concentration inside the bioreactor.
"The morphologies of hand-blown glass vessels function both as lighting and heating elements for the human occupants, and high functioning photobioreactors which provide heat, light, agitation, air supply, nutrient and waste control to the living algae inside," Douenias and Frier explain in their press release.
Given the shortened incubation times along with the small volume of reagents, slide forceps were used to provide continued vertical agitation of the slides in each vial in order to facilitate reagent transfer during the dehydration process.
The experiments demonstrated the successful effect of applying eccentric agitation with UAE to provide a combination of agitation and sonication to extract the proteins in short extraction times.
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