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While not all members of this subfamily have a CBM43 module, its presence would provide affinity for β-1,3-glucans [ 78].
Researchers have been frustrated, however, by apparent context effects on this recognition, i.e., a particular finger will provide affinity and specificity for a given triplet in one sequence but not in others.
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After electrochemical polymerization at 0.95 V in acetonitrile, the resulting polypyrrole film provided affinity interactions with β-cyclodextrin.
In support of the result, Cash et al. (2012) recently reported that FSD3 provides affinity to ligand and stability on perturbations in the ND and FSD2 probably through the interaction of FSD3 of one FST molecule with the ND of the other FST molecule.
Such glucanases may have additional CBMs providing affinity for crystalline surfaces [ 86].
The conserved amino acid motifs of P. pastoris Are2p at positions 224 237 and 406 412 are similar to two sites of S. cerevisiae Are2p providing affinity for oleoyl-CoA [36].
Most likely, there is a combination of factors in the structure of AnMan5B that contribute to high transglycosylation capacity, where R206, analogous to R171 in TrMan5A, might play one of the roles in providing affinity in the aglycone subsites, potentially together with W242.
While alkyl and benzyl substitution provided affinities and antagonist potencies comparable to those of 4-PIOL, diphenylalkyl and naphthylalkyl substitution resulted in marked increase in both affinity and potency.
TRAP provides affinity-based ranking of TFs with a P-value.
As expected, the vinyl groups on the nanoparticles surface provide better affinity between the carriers and luminophor (2,7-NAF.DM) [24].
Privileged structure-based libraries have been shown to provide high affinity lead compounds for a variety of important biological targets.
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