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We report the design, construction and use of an antibody bacteriophage display library built on the scaffold of a single-chain variable fragment (scFv) previously proven to be functionally expressed in the reducing environment of both bacterial and plant cytoplasm and endowed with intrinsic high thermodynamic stability.
The C. crescentus Weimberg pathway was chosen since the corresponding genes had already been integrated and proven to be functionally expressed in the bacteria C. glutamicum and P. putida (Meijnen et al. 2009; Radek et al. 2014).
In addition oral administration of TGF-β has proven to be functionally active in the intestine when using a mouse model [55].
However, currently only several residues in the middle of L4, but not Y538, G540, or S572, were proven to be functionally important for MxA [ 4, 47].
For example, n-loop has been proven to be functionally important in mosquito defensin [ 50] and its change in size will probably influence the activity and antibacterial spectra of these defensins, as observed in navidefensin2-2 [ 25] and nasonin-1.
The aforementioned expression of K17 in the pilosebaceous tract (in the follicular outer root sheath, companion layer, medulla and sebaceous gland; for review, see Langbein and Schweizer 2005) has also proven to be functionally relevant.
Similar(53)
It consisted of several subareas embedded cytoarchitectonically distinguishable and proved to be functionally distinct with some functional overlaps [ 16].
Receptors proved to be functionally active as human and pig chondrocytes attached to RGD-coated surfaces.
In horizons with cohesive character, horizontal porosity proved to be functionally better than vertical porosity.
More than a third of them prove to be functionally illiterate and only half of them graduate on time, while a full 30percentt never finish high school.
However, the results described above suggest that AP-AChE will prove to be functionally more important, at least in the greenbug.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com