Thickness and weight of muscle lesion proved technically difficult to quantitate and was not measured in this study.
The government's reaction was to try to shut down YouTube and Twitter, but this proved technically difficult and legally unsuccessful.
The sealing procedure used for the PDMS chips proved technically difficult with unreliable cellular distribution across the chip and probably lead to the poor long-term viability observed.
Although the behaviorally relevant temperatures for Schistocerca gregaria certainly extend to higher temperatures, stabilizing the preparation temperature with such a differential from the room temperature proved technically difficult.
Finally, if it proves technically difficult to evaluate the morphogenetic potential of the MP-derived cells in these explants, an in-depth molecular profiling of the MP-derived cells in the explants could be performed to establish the identity of these cells at the endpoint of the culture experiment.
In many organisms, this can be very difficult as the identification of ligands for receptors requires expression in heterologous systems and such assays have proven technically difficult.
Despite the importance of these modifications in maintaining cellular homeostasis and contribution to human diseases, identifying which proteins are modified by PTMs in mammalian cells on a proteome-wide scale has proven technically difficult.
"It may prove technically difficult," the doctor told me, "but it can be learned".
Despite the importance of lysine methylation in maintaining cellular homeostasis, the development of proteome-wide approaches for detecting this modification has been limited and has proven technically difficult.
However, they had two concerns that need to be addressed, ideally through specific additional experiments or, if these prove technically difficult, through necessary changes to the text.
