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Although the screening for metastases proved negative for the four patients treated with local excision, their lymph node status and, hence, disease stages were unknown.
The source hatchery for the fish used in this study has remained free of IHNV since its initiation of Sockeye Salmon culture in 1999 (L. Clint, personal communication) moreover viral screening a subset of 60 fish prior to transport to PBS, proved negative for the presence of IHNV using cell culture assay [ 34].
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In addition to these samples, other P. malariae specimens were also tested, but proved negative for PCR amplification of the msp1 gene.
One week later the results of the staining and the cultures were proved negative for bacteria and fungi.
Unfortunately, only one additional DNA sample from an affected relative was available for mutation testing, and this maternal cousin of the index case proved negative for IVS24-48G>A.
In numerous earlier investigations [ 3] detecting just one pathogen in most TD patients, diarrhoeagenic E. coli (e.g. EAEC or EPEC) were not all examined, and even half of the stool samples proved negative for bacterial pathogens [ 3].
In differentiating cells, PFOA also proved negative for effects on cell number, except at the highest concentration, and had no discernible impact on the protein/DNA ratio or the membrane/total protein ratio; however, significant effects were seen for all markers with CPF.
Sera that proved negative for anti-borrelial antibodies were combined to form the NHS pool.
Several sets of 100 μl of SPG solution in the pooled swab samples, which proved negative for C. trachomatis and Ureaplasma, were prepared.
The newly arrived animal treated previously for malaria symptoms proved negative for Plasmodium spp. Both previously released ex-captive, medium-sized male orangutans, brought to OCC&Q from a release site in Tanjung Puting National Park for treatment of amebic dysentery, tested positive for Plasmodium spp. (Table).
All proved negative for anthrax.
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