Rescuing of the uge4 root phenotype proved functionality of the constitutively expressed poplar p35S UGEc and the three Arabidopsis p35S UGE2, p35S UGE4 and p35S UGE5 constructs.
Though lacking the direct repeats reported for NsEPRV [ 1], the 272 to 282 bp conserved box of LycEPRV makes up part of the B1 box that has proved functionality as a promoter-enhancer element for NsEPRV driving GUS expression in apical meristems of A. thaliana [ 15] which suggests a function, possibly as a regulatory element.
The latter was important as before our demo, a still unsolved problem destroyed one of our boards, but we were still able to prove functionality using one transciever board.
The ability to potentially treat multiple individuals through cellular therapy, using primary CEnCs propagated from a single cadaveric donor tissue, can only be realized with a capacity to deliver the expanded CEnCs into the eye, and a proven functionality of the delivered cells to maintain corneal deturgescence, keeping the cornea clear.
Developing therapeutic cell populations from human ES cells will be far more demanding: cells will have to be stable, predictable, pure, have proven functionality, be nontumor-forming, and be "scalable --capable of growing in very large numberscalable --capable
Update: Handshake told TechCrunch it has several potential clients "ready to sign" once beta testing proves functionality — including "a high street bank, a household name telecoms provider and a household name games developer".
During the cruise phase, spacecraft Ka-band telemetry was tracked 36 times by these antennas proving functionality at all antennas.
Nevertheless, as stated above, the only way to prove functionality of a BS is by a series of careful experiments.
We constructed two different versions of STOP (see Methods for details): Δβgeo-3xpA (S1) is a shortened version of a beta-galactosidae/neomycin STOP cassette containing 3 poly-adenylation sites with proven functionality [22], and Neo-2xpA (S2) is a neomycin cDNA followed by 2 poly-adenylation sites.
In vitro experiments showed a direct correlation between the extent of decreasing GSH levels and impaired cell viability (Suppl. Fig. 1), proving functionality and specificity of our approach.
However, results from 1g on ground control samples laid within commercial transport standards, proving functionality and validity of the research equipment and protocols used.
