Heterologous peptides have proven functional in the context of the Ad5 fiber C-terminus and H-I loop as well as the C-terminus of knobless artificial fibers.[9] [13] On this basis, we incorporated each of the four zipper domains into either the C-terminus or the H-I loop of the wild type fiber protein.
The Orc/Cdc6 proteins, experimentally proven functional in their ability to recognize replication origins in other archaea (Pyrococcus abyssi[ 4], Sulfolobus solfataricus[ 5, 6], Aeropyrum pernix[ 7, 8]), were also included in this phylogenetic analysis.
Interestingly, origins within the same family may have different functions among the various haloarchaea, e.g., although belonging to the oriCa family, the active origin in Halobacterium sp. NRC-1 (proximal to orc10) [ 10] was not proven functional in H. volcanii (proximal to orc2) [ 9].
Surprisingly, a specific origin (oriC7-cdc6J) in the minichromosome of H. hispanica, which proved functional, was located in the region with high orthology to H. marismortui.
The analysis approach used identified genes with proven functional roles in leaf development in Arabidopsis and other species, suggesting that our method is biologically robust and the results meaningful.
This handicap, coupled with the limited numbers of experimentally proven functional smORFs in eukaryotes (Kastenmayer et al., 2006; Galindo et al., 2007; Kondo et al., 2007; Hanada et al., 2012; Magny et al., 2013; Pauli et al., 2014; reviewed in Andrews and Rothnagel, 2014), has so far precluded the reliable annotation of smORFs as coding sequences.
ArcA of S. oneidensis MR-1 has been proven functional and involved in the oxygen response as well as in respiration of DMSO and in the detachment of cells from biofilms [ 14, 17, 18].
There's a lot to be criticized about the halfway house of racialized politics, but it has proven functional and stable in this region.
The use of Lyapunov conditions for proving functional inequalities was initiated in [5].
Our findings raise the question about the role of prokinetics when the usual treatment of dyspeptic symptoms is not successful in proven functional dyspepsia cases.
