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F. rubra and F. ovina proved difficult to separate by trnL RFLP since they varied at only 6 nucleotide positions.
This is suggested to be a marker of the integrity of glia and glial transport mechanisms, but the signal has proved difficult to separate from that for the large amounts of glycine present that also resonate at 3.6 ppm [ 236].
However, the alkylation reactions proved problematic leading to the formation of significant quantities of quaternary ammonium salts as side products, which proved difficult to separate from the tertiary amines by column chromatography.
While it proved difficult to separate this pair of thioesters, the mutation G28A was sufficient to suppress thioesterification at this site (Fig. 1(b)) and the His10EPO-1-32(G28A)-MPA thioester was isolated in 28% yield.
Similar(56)
But, according to the article, he grew increasingly uncomfortable with his career choice as it proved difficult to keep his private and NHS work separate.
Neutrality proved difficult to maintain, though.
Alas, it proved difficult to find.
Genocide has proved difficult to prosecute.
The effect proved difficult to measure.
Yet reform proved difficult to implement.
The advice proved difficult to follow.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com