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But diplomats say it has proved difficult to generate much sympathy for grown men who admitted to having worked for Cuban intelligence.
Although two research groups have tried to generate the extracellular domain of TLR4 protein as a decoy receptor for MD-2 [20], [20], it proved difficult to generate a substantial amount of the protein because it is insoluble, its production rate is extremely low, and it is hard to purify due to its intrinsic biochemical properties.
For example, it has sometimes proved difficult to generate drug-resistant P. falciparum mutant clones in vitro [ 8] and in these cases it has been necessary to await the appearance of resistant parasites in field samples.
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That way, even if revenue proves difficult to generate, there is always the possibility of another way to cash in — a takeover.
In this study, we engineered K. marxianus to produce and secrete a single-chain antibody (scFv), a product that is highly valuable but has historically proven difficult to generate at large scale.
Thus far, it has proven difficult to generate mature myofibers in vitro with T-tubules transversally paired with SR in striated transversal triads and peripheral nuclei (Flucher et al, 1991; Cooper et al, 2004; Cusimano et al, 2009).
Genocide has proved difficult to prosecute.
The effect proved difficult to measure.
Neutrality proved difficult to maintain, though.
Alas, it proved difficult to find.
Yet reform proved difficult to implement.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com