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The 5' end of most viruses has also proved difficult to complete and we have found that the addition to the RT reaction of degenerate oligos based on conserved 5' sequences can increase coverage.
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At the same time, American analysts say that India's military spending process is so chaotic and unfocused that it is proving difficult to complete deals.
These latter pieces were proving difficult to complete as Lutosławski struggled to introduce greater fluency into his sound world and to reconcile tensions between the harmonic and melodic aspects of his style, and between foreground and background.
The pyrene derivatives proved difficult to dissolve completely and a number of heating and cooling cycles were undertaken to ensure complete dissolution and homogenisation occurred.
These tests were completed for seven of the mutants because tgr4, tgr5, and tgr8 proved difficult to propagate due to reduced seed set and low germination frequencies.
Genocide has proved difficult to prosecute.
The effect proved difficult to measure.
Neutrality proved difficult to maintain, though.
Alas, it proved difficult to find.
Yet reform proved difficult to implement.
The furlough plan proved difficult to enforce.
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