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We furthermore used different in vitro assays to prove the activity of the protein after particle binding and showed that tPA is still active after coupling to the particles.
Initially, nonelectrochemical control experiments using alcohol dehydrogenase-containing alginate gel beads and the coenzyme NADH were performed to prove the activity of the enzymes.
To prove the activity of vitamin D in protecting against UTIs, Hertting and colleagues demonstrated a significant increase in the production of cathelicidin after vitamin D3 supplementation in bladders infected with uropathogenic Escherichia coli.
These favourable results, although still do not prove the activity of irinotecan in these patients since the two other drugs, oxaliplatin and paclitaxel, have been shown to be active in refractory germ cell cancer (Bokemeyer et al, 1996; Kollmannsberger et al, 2002a).
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The metabolites identified, 6α-OH-T, 6β-OH-T, 15β-OH-T, and 4-AD, after exposure of bovine liver slices to T prove that phase I enzymes are active, and the formation of T-17- d-glucuronide proves the activity of at least one phase II pathway.
Collagen and gelatin zymograms, in which the proteolytic enzymes were separated from TIMPs before the assay, proved the activities of collagenase MMP-1 and gelatinase MMP-2, as dosed in conditioned media, were higher in desmoid than in normal fibroblasts.
Moreover, behavioural simulations prove that the activity of the A-ADC is reduced by two orders of magnitude compared to its synchronous counterpart.
To prove that the activity of caspase-3 is reduced after downregulation of ROCK2, we further performed a western blot analysis against cleaved (i.e., active) caspase-3.
Recently, we used calcium imaging techniques to record dozens of neurons simultaneously with single-cell resolution to prove that the activity of the striatal microcircuit recorded in vitro during dopamine (DA) depletion is radically different than that recorded in the controls [ 3].
To further prove the biological activity of the candidate compounds, tumor xenograft studies were performed in SCID mice (Fig. 6).
Other reports also prove the antitumoral activity of H1 histamine receptor antagonists in human leukemia, myeloma [ 4, 5], colon, and liver cancer cells [ 6].
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