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Lamellipodial kymographs show pixel densities over time (x-axis) on a single pixel-wide straight line (y-axis) in the direction of lamellipodial protrusion (Image J plug-in multiple kymograph).
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The principle of LifeViz "Absolute" is the measurement of a cavity or a protrusion using simple image acquisition.
High magnification images of acini indicated E-cadherin was associated with cellular protrusions at the basal membrane and that desmosomal staining was characteristically punctate but was not associated with protrusions (Figure S2A and S2B).
a Sagittal T2-weighted image shows protrusion of a thinned and dehydrated intervertebral disc (Pfirrmann IV, arrows) at L5-S1.
T2-weighted, fat-saturated axial MR image shows protrusion of the implant shell through a focally weakened part of the fibrous capsule at medial aspect of right breast, simulating a rat tail (arrow) 3.
Abdominal computed tomography (CT) showed a bilobed image with protrusion through the umbilicus, with attenuation coefficients of 3, 7 and 45 HU; after administration of contrast enhancement they reached 6, 16 and 88 HU, with a size of 68x60x76 mm.
Compared to the bare tip STM image, each bright protrusion splits into two protrusions with inequivalent brightness in the CO-STM image in Fig. 2h.
In high-resolution DCL cell image recordings, protrusions and cell tracking were quantified by segmenting the DCL cell body followed by segmentation of the DCL cell membrane.
Maximal filopodia length was measured using ImageJ software, and filopodial duration was determined by persistence of the protrusion through time-lapse images.
The finger-like protrusions of live imaged enteroblasts also contained a dense network of microtubules, whereas the lamella-like structure was devoid of filamentous tubulin (β-tubulin-RFP in Fig 2F H), typical distribution of microtubules in migrating cells (Wadsworth, 1999).
Membrane protrusion analysis was done using Image J software and based on a program described elsewhere [ 29] with slight modifications.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com