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CO oxidation is a prototype reaction for studying oxidation of small organic molecules.
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Once a prototype reaction is selected, an in-depth mechanistic analysis is essential to guide the requisite adaptations for use in biological systems and to anticipate potential pitfalls.
For this reason, rate enhancement is a common initial goal in transforming a prototype reaction to a bona fide bioorthogonal transformation.
(v) This adjustment to the prototype reaction was sufficient to generate a bioorthogonal chemical reaction.
In our experience, the chemical literature from the early to mid-20th century is fertile ground for unearthing prototype reactions.
The handful of prototype reactions on which current bioorthogonal transformations are based were discovered long before the chemistry/biology interface was a fashionable venue for research.
The Staudinger reduction appeared well-suited as a prototype for bioorthogonal reaction development because the two participants were abiotic, mutually and selectively reactive, mostly unreactive with biological functionalities, and tolerant of water.
The prototype for such reactions is conjugation with glutathione (GSH), which occurs spontaneously and enzymatically, leading to formation of thioethers and mercapturic acids.
In conclusion, we have demonstrated the utility of TX-TL reactions for rapidly prototyping and characterizing RNA circuitry.
To find a prototype for the overheated reaction to the health care bill, you have to look a year before Medicare, to the Civil Rights Act of 1964.
A prototype capture device for the 5184 reaction well chips has been used in some preliminary testing, but for this study we have used a 4 quadrant chip layout (841 wells per quadrant, see Figure 1) and the matching disposable extraction fixtures to perform target capture of up to 4 samples on a single chip.
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