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/p proton balance.
This uncertain algebraic system was dissolved following Hensing et al. [39] by adding a proton balance and introducing the yield coefficient (Y X/S informs about the relation of assimilatory to dissimilatory substrate utilization and allows to establish the mass ratio between formed biomass and consumed lactose).
These experiments showed that the regulation mechanisms for the proton balance were indeed functioning.
Neutral formulas obtained from PubChem http://pubchem.ncbi.nlm.nih.gov/ were used to validate reaction stoichiometry (mass balancing) including proton balance.
Therefore, further refinement of the proton balance involves controlling the dynamics of nitrogen availability in addition to the stoichiometric composition.
Therefore, the nitrogen ratio that will achieve a proton balance will be dependent upon the level and composition of products formed.
Similar(45)
It was not possible to properly simulate using flux balance analysis due to improper proton balancing.
Interestingly, it turns out that the intercellular transport of metabolites is not properly proton balanced.
All transporters and lumped reactions were redone with proper elemental and proton balancing.
A bicarbonate buffer reaction, similar to the function of the kidney, was added to account for proton balancing.
The results in this study clearly highlight the ability of the genome-scale models that incorporate detailed proton balanced biochemical reactions to predict, albeit qualitatively, the proton exchange rates and other aspects of physiology.
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