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All the protocols were initiated by a hot start (75°C, 2 min) prior to addition of rTth enzyme.
Acute and chronic dextran sodium sulfate (DSS) treatment protocols were initiated on six week old female C57BL/6J mice (Jackson Labs) maintained on standard AIN-76A rodent diet.
Due to unsatisfactory results, intensified radiotherapy and concurrent radiation and chemotherapy protocols were initiated [ 3].
Head ROI imaging protocols were initiated 2 hours after tracer injection, allowing 3 4 circulation half-lives; therefore, measured head fluorescence concentration was primarily indicative of the brain-accumulated/retained tracer, with small contribution of circulating tracer.
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The PCR protocol was initiated at 95°C for 10 min for initial denaturation of the cDNA and hot-start Taq-polymerase activation, followed by 45 cycles of a two-step amplification programme (15s at 95°C; 40 s at 60 64°C), according to the primer set used.
Therefore, the current protocol was initiated at the time of the onset of initial Phase II studies.
After electronic correspondence and several perioperative leadership meetings, the enhanced pediatric Universal Protocol was initiated.
After this challenge, preparations were left undisturbed for 30 45 min to stabilize before the experimental protocol was initiated.
The department's "active shooter protocol" was initiated, he said, leading to the evacuation of the hospital.
She will show that distance was maintained through careful construction of the research questions and protocol so that there was no room to insert herself into the study once the protocol was initiated.
Therefore, it was concluded that Presynch-Ovsynch protocol could be more reproductive and beneficial when a single treatment with PGF2α was administered at 15 days postpartum (15 days after the PGF2α, Presynch-Ovsynch protocol was initiated).
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