Contemporary protocols utilise a combination of transurethral tumour resection, concurrent radiation and radiosensitising chemotherapy, and often adjuvant chemotherapy.
14 This strategy will be supplemented with additional community-based follow-up protocols utilising an existing CHW network and hospital-based quality improvement mechanisms.
The recently launched Nugen and Nugen FFPE protocols utilising both oligo dT) and random priming are therefore especially promising for FFPET substrates (Nugen Technologies, CA, USA) and are likely to significantly improve the sensitivity of FFPET arrays.
There should also be a discussion between all concerned as to whether, or how, this principle could be extended to less commonly used medicines where ULM are part of evidence-based protocols utilising good, published, and audited evidence of safety and efficacy as described by Mellor and colleagues [ 20].
The regional protocol utilised cylinders with contact surfaces of 3.14 and 15.9 cm2 to apply pressures of 50 and 200 kPa.
The imaging protocol utilised, as well as the anthropomorphic phantom, is suitable for different SFOV gamma imaging systems and the experimental setup can be replicated for comparison purposes.
The first protocol utilised the High Pure FFPE RNA Micro Kit (Roche, Mannheim, Germany) and the second protocol utilised TRIzol reagent.
Assuming that the protocol utilised during the ALMANAC study was continued, the 25% of cases with positive lymph nodes would require a second operation to clear the axilla.
As such, the screening procedure protocol utilised in this analysis (referred to as 'EDCTS Screening Protocol') may not precisely reflect the I-ELCAP protocol or screening procedures presented in I-ELCAP studies.
The brain imaging protocol utilised sagittal, dorsal and transverse T2-weighted (TR/TE 5190/108 ms) and T1-weighted (TR/TE 386/13 ms) sequences and transverse FLAIR (TR/TE/TI 9110/122/2500 ms) and gradient echo (TR/TE 1000/28 ms) sequences.
