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These data do not fulfill in all aspects the pre-conditions of scientific mixture toxicity concepts but are characterized by a considerable degree of data heterogeneity due to variation among laboratories, test protocols, test species, and measured endpoints even when a considerable effort is made to select toxicity data that were derived in similar tests (first data set).
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The PCR protocols tested are summarized in Table 1.
The first two protocols tested provided valuable lessons.
The final two protocols tested in this study perform protein digestion in solution.
The standard denaturation and extension temperatures and times were retained in the four 'touchdown' protocols tested.
The three other 514 nm light protocols tested were found to be less effective than the 630 nm regimen.
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· Super Size Me is out in September A Toxic Screen Protocol test costs £250.
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