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Furthermore, experimental factors such as difference in probe sequences, RNA handling, hybridization protocols, scanning, and image analysis are likely to contribute to the observed divergence.
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In particular, each reader separately runs the enhanced identification protocol scanning its own interrogation region.
The experimental protocol, scanning, image processing and data extraction was carried out as previously described according to the instructions of the manufacturer [23], [53], [54].
This protocol scanned a 6 × 6 mm area centered on the optic disc.
A modified protocol scanning along the midclavicular line, anterior-axillary, and posterior-axillary lines described by Lichtenstein and Meziere was used in case 2 due to critical illness[8].
MitoChips were hybridized overnight, washed on the Affymetrix fluidics station using the pre-programmed CustomSeq™ resequencing wash protocols, and scanned.
DXA scans are obtained with a fan-beam scanner, Delphi A™, Hologic (Waltham, MA), using manufacturer's recommendations for patient positioning, scan protocols, and scan analysis.
The median CTDIvol of the reference protocol scans was 7.8 mGy (CI 6.8 8.4 mGy).
For subjective image quality, three 80 kVp, 170 mg I/mL study protocol scans with median CTDIvol of 7.1 mGy were considered (Table 4, Fig. 5).
The ONHV was determined from a custom protocol scan which generates 145 B-scans focusing the optic nerve head at an angle of 15° × 15° and a resolution of 384 A-scans per B-scan (ART = 10).
Probing and scanning protocols have been detailed elsewhere.
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