Sentence examples for protocols pierce from inspiring English sources

Reactions were developed with an enhanced chemiluminescence system, according to the manufacturer's protocols (Pierce).

The experimental execution and processing of results were based on previous protocols (Pierce et al., 2007).

Nuclear and cytoplasmic extracts of synovial fibroblasts were prepared using NE-PER nucleandand cytoplasmic extraction reagents according to the manufacturer's protocols (Pierce, Rockford, IL, USA).

The UFBP1 peptide was conjugated with maleimide-activated mcKLH following the manufacturer's protocol (Pierce).

After 24 h, the nuclear proteins were extracted according to the manufacturer's protocol (Pierce).

Bound antibodies were visualized with a chemiluminescent substrate, following the manufacturer's protocol (Pierce, France).

Where indicated, peptides were labeled with DyLight 488 or 649 fluorochromes according to manufacturer's protocol (Pierce, Rockford, IL).

Protein concentrations were determined using the Bicinchoninic Acid (BCA) protocol (Pierce, Rockford, IL), loaded on 10% Bis-Tris Gels (Invitrogen, San Diego, CA), and then processed for Western blotting.

Co-immunoprecipitation experiments were performed using the ProFound Mammalian HA Tag IP/Co-IP and c-myc Tag IP/Co-IP kits following the manufacturer's protocol (Pierce, Rockford, IL).

OxP-22 was obtained by trimerizing OxP-20 in 50% aqueous solution of DMF using Tris-[2-maleimidoethyl]amine and the manufacturer's protocol (Pierce Biotechnology, Rockford, IL, Cat#33043).

For immunohistochemistry, patient and normal sera (300 µl) were purified using the Melon Gel IgG purification kit according to the manufacturer's protocol (Pierce Biotechnology, Rockford, IL) to remove IgM, and purified sera were concentrated by Amicon Ultra 100 (Millipore, MA).