Sentence examples similar to protocols of expression from inspiring English sources

The obtained sequencing data were analyzed for: library complexity and reproducibility; rRNA removal efficiency and bias; the number of genes detected; coverage uniformity; and the impact of protocols on expression biases.

After amplification, each labelled cRNA or cDNA target was biotinylated, fragmented and hybridised on Affymetrix HG U133 plus 2.0 to assess the impact of each protocol on expression profiles.

The chemostat compendium allows us to adequately model the influence of the hybridization protocol on expression.

In addition, strategies and protocols for expression and delivery of ribozyme genes are addressed.

Expression vectors and protocols for expression and purification of the following protein fragments in E. coli were described previously: rat syntaxin 1A residues 191-253, rat synaptobrevin 2 residues 29-93, human SNAP-25 residues 11-82 and 141-203, full-length rat CpxI and rat CpxI residues 26-83 (Pabst et al., 2000; Chen et al., 2002).

While blockage of globin transcripts during first strand cDNA synthesis with globin PNAs resulted in the best overall performance in this study, we conclude that selection of a protocol for expression profiling studies in blood should depend on several factors, including implementation requirements of the method and study design.

E. coli DHFR was expressed and purified using a similar protocol for expression and purification of B. anthracis DHFR, as we described previously in [30].

In addition to presenting an optimized protocol for expression and purification of PglB, these results present a general guide for the systematic optimization of the expression, purification, and stability of a large, transmembrane protein.

Here we describe a protocol for expression and purification of mature human VEGF-D, and a mutant thereof with reduced glycosylation, potentially suitable for preclinical therapeutic and structural studies, respectively.

The amplification products were used as a microarray target to optimize a protocol of gene expression profiling.

However, these modifications in GABA-T properties promoted by the protocol of DRF/FEO expression can be considered as a metabolic adaptation based on the biochemical plasticity of this enzymatic system.