Information (such as protocols, instructions, and reference books) should be geared to the practical tasks of preparing and administering antimicrobials.
The entire protocol, instruction and training included lasted about an hour.
Sequencing reaction setup was performed in a final volume of 10 μL with Big Dye Terminator kit v 3.1 Chemistry (Applied Biosystems, Paisley, United Kingdom) following protocol instruction.
As the number of ODs increased, or patients were receiving mechanical ventilation or vasopressors, or had recent surgery (probably a result of protocol instruction to commence or resume DrotAA infusion only 12 hours or more after surgery), the time to treatment increased.
This two day event is a requirement in the application process were applicants are exposed to classroom, physical fitness and protocol instruction.
Incoming festival director Clare Stewart met the Queen last week but forgot the protocol instructions palace staff had given her — nervous as Her Majesty appeared before her, Clare just curtsied and said "Greetings".
We could not assess whether trial staff adhered to protocol instructions, which may have impacted PRO compliance rates.
Supernatants yielding extracellular bacteria were collected, diluted according to protocol instructions, and plated on P. aeruginosa isolation agar (BD Diagnostics Sparks, MD, USA).
First generation of α7+/+ and α7−/− animals were distinguished through genotyping following JAX-protocol instructions.
The pooling, DNA fragmentation, labeling, and chip hybridization were performed according to Affymetrix CustomSeq Resequencing protocol instructions.
