Using the co-evolution-guided structure prediction protocol described above, we generated models for the structures of CydA and CydB, and then docked the subunits together using inter-protein predicted contacts as described in Ovchinnikov et al. (2014) to generate a model for the entire TM complex.
Using a combination of experimental and computational techniques, the paper provides a general protocol for studying the structure in solution of molecular systems characterized by the existence of conformational metastable states.
The requirements of simple and reliable protocols for the synthesis of anisotropic structures with controlled morphology continue to be a major challenge in nanoscience.
The protocol itself draws heavily from Japanese Zen Buddhist philosophy and the structure of Japanese tea ceremonies, placing special emphasis on spatial emptiness and ritualized behavior.
It illustrates the structure of the Observation class: Data, Events, Protocol and State, as well as Description for meta-information.
Additionally, the structure of the software created limitations in capturing nuances of complex contact tracing protocols.
The structure of the model along with all parameter definitions are delineated in Protocol S1.
Assay protocols, structures of tool compounds, double-titration experimental data with 25, BODIPY-cyclopamine competitive binding assay data, and protocols for the preparation of 25 and 29.
Here, we describe our recently employed protocols for determining the high-resolution structure of the virus-like particle of hepatitis E virus (HEV), a pathogen of viral hepatitis in human.
