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Variation in the apparent therapeutic efficacy may have resulted from a lack of reproducible, standardized protocols for preparation.
However the complex protocols for preparation of eukaryotic cell-free protein expression systems result in high manufacturing costs and limit their utility.
The detailed protocols for preparation are described in Meyer et al. [45], [46] and the manufacturer's instructions (GS FLX platform, Roche).
Most importantly, there is a need to establish efficient protocols for preparation of primary cell cultures from human tissues and to establish the nucleofection efficiency of related genes into such cell cultures.
Protocols for preparation of the [C]-benzoyl-CoA, 2,4-dihydroxyphenacyl-CoA, and phenacyl-CoA.
Drugs and fluids require safe handling by anaesthetists, who should follow local protocols for preparation and administration to prevent contamination.
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In this study an automated protocol for preparation of samples prior to massively parallel sequencing is described to prepare DNA for paired end sequencing on the Illumina HiSeq 2000 instrument.
A protocol for preparation, culture and transfection of primary rat embryonic E18 hippocampal neurons and determination of neurite length was adapted from [68].
The protocol for preparation of passivized glass surfaces for single-molecule transcription measurements will be described in detail elsewhere.
Paired-end library preparation was performed at the European Molecular Biology Laboratory EMBLL) Genomics Core Facility (Heidelberg, Germany) using the Illumina protocol for preparation of genomic DNA sequencing libraries.
The synthetic protocol for preparation of 1-C-phosphoenolpyruvate d2, precursor for parahydrogen-induced polarization (PHIP) of 1-C-phospholactate d2, is reported.
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