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This paper studies the state of the art of current real-time solutions including MAC protocols, routing protocols, data processing strategies and cross-layer designs.
We suggest that the major contributions to poor correlations are attributable to either platform specific differences in protocols, data processing or differences inherent to each technology.
Although the manufacturers have developed platform-specific quality controls, they are often not adequate for cross-platform comparisons, particularly for the evaluation and standardization of transcriptomic data due to differences in protocols, data processing and analysis methods.
The development of a wide range of technologies and platforms for measuring mRNA expression makes the evaluation and standardization of transcriptomic data problematic due to differences in protocols, data processing and analysis methods.
Further, acquisition protocols, data processing methods and pipelines optimized for use in vivo are unlikely to perform well post-mortem without careful adaptation (Pfefferbaum et al., 2004; Miller et al., 2011).
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visits 1, 2, 3, 4. VP: creation of the study design, protocol, data processing and interpretation.
Other parameters, including array and experimental design, protocols and data processing procedures, can affect array data independently of biological processes.
Due to the different microarray platforms (Affymetrix, Agilent Technologies), experimental protocols and data processing for normalization used by authors, we choose to use gene expression raw data individually.
We conclude that the knowledge acquired from early work on the technical issues related the protocols and data processing methods applied to gene expression microarrays have greatly facilitated the rapid implementation of highly-reproducible data using miRNA arrays.
In addition, we have noted several large scale comparison studies, such as the MAQC-III multi-community efforts [ 12], the ABRF next-generation sequencing study [ 13], and Wang et al. study [ 14], but these have been primarily focused on the accuracy and reproducibility of multiple RNA-seq systems, protocols, and data processing pipelines.
The full description of the Extraction protocol, labeling and hybridization protocol and data processing is obtainable in the GEO DATA base under http://www.ncbi.nlm.nih.gov/geo/ (accession number GSE14277).
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