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RNA was purified by pegGOLD (peqLab, Erlangen, Germany) using standard protocols, and complementary DNA was generated from 1 mg RNA using a complementary DNA synthesis kit (Roche, Basel, Switzerland).
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When microsatellites are isolated using standard SSR enrichment protocols and primers designed at complementary repeated regions, amplification from multiple genomic sites can cause scoring difficulties that compromise their utility as markers.
The Delphi technique has successfully been used to reach consensus in medical and health services research [ 31], and complementary medicine treatment protocols, including yoga for musculoskeletal conditions [ 32], and acupuncture [ 33- 35].
Poly-A RNA isolation, RNA fragmentation, and complementary DNA (cDNA) synthesis protocols were performed as in Yoon and Brem (2010).
As depicted in Fig. 13, the architecture is able to handle several industrial protocols and able to connect to complementary SCADA elements, such as additional PLCs and RTUs.
The way in which DCD procedures are described varies, from no specific protocol (two countries) to detailed protocols with complementary consensus statements and ethical frameworks, as in the UK [ 17].
A typical treatment protocol for Jane (listed alphabetically below) combines these with adjunct and complementary therapies for improved healing.
A standardized feeding protocol was applied that defined feeding increments, handling of feeding difficulties, and complementary parenteral nutrition.
Isolated RNA was quantified using the ND-1000 spectrophotometer (NanoDrop) and complementary single strand DNA was synthesized using the Omniscript RT Kit according to the manufacturers protocol (Qiagen).
Avoidance of unprotected intercourse and receipt of antiretroviral treatment by the infected partner in accordance with protocols are complementary measures to prevent HIV transmission.
"We have complementary missions and complementary collections".
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