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Differentiation protocols and cell culture conditions were as detailed in Ma et al. 2011.
This review focuses on recent findings relating to experimental protocols and cell penetrating peptide modifications or extensions that yield significant effects on penetration capability.
The combination of appropriate differentiation protocols and cell culture polymeric biomaterials for the differentiation of hPSCs into specific cell lineages will produce a large quantity of highly pure GMP-grade differentiated cells for use in translational medicine.
This may be partly due to chance and in some cases due to artifacts (i.e., product formed during processing or due to methodology; Brusick, 1986; Galloway et al., 1991), differences in experimental protocols and cell lines, use of high versus low concentrations and doses, or improper interpretation of the data (Green and Muriel, 1976).
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For EB formation in hanging drops, ES cells were trypsinized and counted using standard protocols, and cells were either resuspended in medium without or with CTX (1 µg/ml).
Animals were euthanized by approved protocols, and cells from individual mice were collected for flow cytometry.
Using the same immunosuppression protocol and cell dose, we previously reported NSC survival 8 weeks post-transplant in APP/PS1 mice24, and in our preclinical ALS studies we have shown human spinal stem cell survival in similarly immunosuppressed rats47,49.
To address this issue, we combined a murine model of corneal abrasion with pharmacological tools, a restraint-stress protocol, and cell phenotypic and transcriptional analyses for corneal macrophages to determine the influence of the ANS on the murine corneal wound-healing process.
The cell viability percentage was determined according to the kit protocol, and cell viability percentage was plotted on a graph.
We have generated iPSCs from a panel of seven individuals using a consistent protocol and cell type of origin.
Protein concentration was determined according to the manufacturer's protocol, and cell extracts were equalized to a protein concentration of 1.0 mg/mL for the assay.
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