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We present an experimental method for measuring "realistically" the swelling ratio in terms of weight (SRW) of PDMS, where "realistically" means without the underestimation involved by the standard protocol (weight-measurements in open environment with time-delay incompatible with fast solvent evaporation rate).
At the beginning of the protocol, body weight was similar between the groups (~230 ± 15 g).
Protocol 2: Weight of mice should be recorded at day-0/day of injection.
At the beginning of the protocol, body weight was not statistically different between study groups (C: 280 ± 17; D: 298 ± 10; MI: 313 ± 4; DMI: 271 ± 4.5 g).
At the end of the protocol, body weight gain, adipose tissue content, serum lipids and glucose levels, and aorta morphometric and biochemical measurements were performed.
Other endpoints not initially included in the protocol are weight gain during the first 8 days of life and the time from BCG vaccination to the appearance of a scar.
It remains part of the standard autopsy protocol that weights are obtained for all major organs, although the clinical significance of organ weights for determining cause of death in this infant population remains uncertain.
Both protective protocols limiting weight bearing and accelerated which do not, have shown clinical success.
During this stage of the treatment protocol, full weight-bearing, with no more than a single crutch, was advocated.
The post-operative protocol comprised weight-bearing as tolerated and physiotherapist-based inpatient then outpatient exercise programmes.
The protocol employed weights the residuals arising from each of these classes equally, given the number of datapoints available for each.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com