Similar(8)
For transfection of 80 cm2 flasks the protocol was scaled up to 9 ml of media and cellular density to 6×105 proportional to the increased surface of adherence and optimised to have more than 70% confluence after 24 h in culture.
For purification, this protocol was scaled up to 30 ml starter cultures and 400 ml expression cultures.
From Erlenmeyers to packed bed bioreactor the process was scaled-up 10 times.
The process was scaled-up successfully in the pilot plant to manufacture material for clinical supply.
Also, the aqueous two-phase system was scaled-up 100 times.
An existing photobioreactor design was scaled-up from 9 L to 20 L.
The process was scaled-up to treat 2 kg of biomass.
It suggests not only that S. bacillaris strain siva2011 biomass was scaled-up, but also that the TAG production metabolism appeared to be scaled-up as well.
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