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The cell nuclear fractionation protocol was performed as previously published59.
Standard transfection protocol was performed and described in the Supplementary Methods section.
Standard immunohistochemistry protocol was performed on paraffin sections used with antibodies listed in the Supplementary Table 1.
Endoscopic tracheal clipping according to a standardized surgical protocol was performed in all animals.
A combined analysis of four randomised trials using a core protocol was performed.
This protocol was performed by six subjects and recorded using an 8 × 8 HD-sEMG grid.
The cementation protocol was performed according the manufacturer's instructions.
Seven or more days later, the same MRI protocol was performed after intravenous injection of 10 mumol/kg MnDPDP.
A written, signed and dated ICF was provided before any protocol was performed.
The study protocol was performed in four sequential stages (Figure 1).
ETT, following the standard Bruce protocol, was performed by specialized ETT laboratory personnel.
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