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The effectiveness of this protocol was confirmed by comparing the responses of Japanese high school students on questionnaires completed before and after participating in the experiment.
The selectivity of the extraction protocol was confirmed by analyzing some elements (Ca, C, Fe, Mn, Si and Al) designed as indicators of the targeted phases.
The efficacy of the degassing protocol was confirmed by analysis of Davisil LC250 (Sigma-Aldrich Corporation, St. Louis, MO, USA) for which the surface area, pore volume and mean pore diameter data repeatedly agreed with the manufacturer's specification.
The effectiveness of our isolation protocol was confirmed by the characteristic "cobblestone shape" of isolated cells at confluence as well as their expression of all the examined endothelial markers.
The aggregation protocol was confirmed by the low-shear viscometry measurements (Fig. 5).
The efficiency of the current protocol was confirmed by the following observations.
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The rightness of the protocol is confirmed by the agreement with experimental data.
Specificity and sensitivity of the anti-KIT antibody and efficacy of the staining protocol were confirmed by the relevant staining patterns observed in the characterized KIT positive and negative control tissues.
One patient who was reported as having lung metastasis on both protocols was confirmed with primary lung cancer on biopsy.
General agreement between the two genotyping protocols was confirmed in frogs analyzed with both protocols because they were caught in both time periods.
Two additional genes, HLA-DR alpha and SNRP-B, that were also obtained using the two protocols, were confirmed by northern blotting (Fig 2A).
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