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The original real-time RT-PCR protocol was complemented by the addition of 200 pmol/μL of internal control probe YFP2 (5′-ROX-ATCGTTCGTTGAGCGATTAGCAG-BBQ-3′).
Using ubiquitin as a case study, the computational protocol was complemented by experimental validation and was shown to yield selective binding by means of a conformational shift due to designed single-residue mutations.
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Analysis is carried out by quantitative EM, with conventional fixation/staining protocols being complemented by cryo-EM following high-pressure freezing.
The protocols from processing and deciding upon the simulated cases (Think-Aloud Protocols) were complemented by protocols from a task in which the doctors were asked to describe how they usually reason in their practice when drug prescription for elevated cholesterol values may be indicated (Free-Report Protocols).
The protocol for RNA isolation described here was complemented by adding a separate demodification step which involves incubation at elevated temperature in a buffer containing ammonium chloride which favors the reversion of methylol groups to amino groups in nucleobases.
The olsA mutation was complemented following the same protocol by introducing plasmid pYLI-1 into the BABΔolsA mutant.
The CSMA/CA MAC protocol can be complemented in such scenarios by interference cancellation (IC) algorithms at the physical (PHY) layer.
Therefore, to detect migrants with latent infection the current screening protocol may be complemented with more sensitive techniques such as interferon- γ assays reportedly having estimated sensitivity: 80 95% and specificity: 95 100% to detect latent M. tuberculosis infection [ 45].
We, therefore, argue that simulation-based evaluation of ad hoc routing protocols should be complemented with mathematical verification and comparison.
Results generated by proteomic protocols should be complemented by preclinical testing of antivenom efficacy using functional neutralization assays.
A range of new user interfaces and systems have been developed as novel and relevant tools in neurorehabilitation, allowing the implementation and exploration of new rehabilitation approaches and protocols to be complemented and to improve upon traditional methods.
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