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Adherence to the treatment protocol was ascertained through the use of the written therapist guide, the structured evaluation sheet, and the self-help manual and supervision of therapists by an experienced clinician (SJ).
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The clinical samples were derived from prospective trials where data collection, validation, and processing followed good clinical practice; the adherence to study protocols was ascertained and a continuous follow-up for nine years sufficiently substantiates our observation.
Studies published from 2005 to 2014 with a prespecified protocol (how AF was ascertained, diligence with which medical records were sought, modes of clinical assessment, and follow-up of patient subgroups) analyzing the epidemiology of AF exclusively in the general population were considered.
Operation time was ascertained from the operation protocol.
The quality of the mouse RNA was ascertained using the Agilent Bioanalyser 2100 using the NanoChip protocol.
Reaction specificity was ascertained by performing the Melt® procedure (58 99°C, 1°C/5 s) at the end of the amplification protocol, according to the manufacturer's instructions.
Specificity of each reaction was ascertained by performing the Melt procedure (58°C to 99°C; 1°C/5 seconds) after completion of the amplification protocol, according to the manufacturer's instructions.
Once the quality of the fosmids was ascertained, sequences were determined using Roche 454 GS FLX Titanium technology, according to the manufacturer's protocols (Roche Applied Science, Indianapolis).
Mortality was ascertained through verbal autopsies.
Mortality was ascertained in 2007.
Adherence to the study protocol will also be ascertained by measurement of omega-3 fatty acid concentrations in maternal plasma at baseline and at 34-36 weeks' gestation.
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