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We develop an experimental protocol to visualize decrypted images that otherwise would have been barely recognizable, while keeping the standard security levels.
Transmitted light images were obtained after H&E staining as per standard protocol to visualize the morphology of the sections.
A Vectastain Elite ABC kit (Vector) was used according to the manufacturer's protocol to visualize bound rat antibodies.
The next day sections were subjected to normal day 2 immunofluorescence protocol to visualize Iba-1 (described above).
The Super Signal WestFemo Maximum Sensitivity Substrate (Thermo Fisher Scientific) was used according to the manufacturer's protocol to visualize protein expression and the band intensities were quantified by the ImageJ software.
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Silver staining protocol was employed to visualize protein spots [38].
After the first visit patients were referred to a 3.0 Tesla MRI scan performed according to a special protocol [11, 12], designed to visualize the trigeminal nerve and its relations, and to identify symptomatic trigeminal neuralgia.
Several protocols have been used to visualize host cytoskeleton interaction with parasites using EM.
The substrate ELC plus (GE Life Sciences) was used to visualize staining according to the manufacturers protocol.
Vectastain ABC Kit and DAB substrate Kit (Vector labs) were used to visualize the signal following the manufacturer's protocol.
To visualize flagella, we used a fluorescence labeling protocol developed by Turner et al. (2010).
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