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Two primary procedural challenges emerged during the present study that should be better addressed in the future: one is the need for all of the components of the protocol to be completed at all sites, particularly sampling of all depth strata, replicates and target organisms.
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Therefore, room temperature was chosen for the subsequent experiments, since the integrity of the isolated cells was preserved for longer periods than at the higher temperature, allowing the more lengthy voltage clamp protocols to be completed.
As per protocol, the HRQOL questionnaires had to be completed before knowledge of treatment allocation by the patient (up to 2 weeks before randomisation), at cycle 2 just before the next cycle (at the time of tumour assessment), at the end of CT before starting RT (at the time of tumour assessment), and then, 6 and 9 months after completion of RT.
The EGDT protocol comprised a set of tasks to be completed within the first 6 hours of presentation, including the placement of a central venous catheter to monitor hemodynamic variables during fluid resuscitation.
The reconstruction of a metabolic model is a laborious and extensive process that has been described by Thiele and Palsson in 2010 [ 49] as a 96 steps protocol, which takes a long time to be completed, depending on data availability.
Our protocol dedicated that the measurements had to be completed between 6 and 16 h after stroke symptom onset, mimicking the period of early in bed mobilisation initiation during daytime.
The optimized detection protocol could be completed within 1 h.
A think aloud protocol may be completed by the novice during task performance.
The imaging protocol could be completed in 39 patients.
As a major contributor to the systematic review literature, we believe that all Cochrane protocols should be completed and published as Cochrane reviews.
While the microdevice architecture described here is complex, these protocols could be completed using a less automated system.
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