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For the HybMap protocol, probe values were median scaled to the median intensity from the zebrafish control probes.
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Each protocol probes different aspects of the nonlinear viscoelastic behavior of the material and a combination is useful to obtain a complete description (Semmrich et al. 2008; Broedersz et al. 2010).
Detailed protocols, probe sequences and mappings, gene annotations, all microarray data, and information about the microarray hybridizations are posted on the project website [ 55].
Detailed protocols, probes and the antibodies used are described in the supplementary Materials and Methods.
In principle either SYBR Green based protocols or probe based protocols can be used for Real-Time PCR.
Major results: There was a difference in task demand as measured by reaction time between the two protocols (the multiple probe protocol was more demanding), and a difference trend in P300 detection sensitivity between protocols for both information types combined in favor of the 1PB (p < .07).07
Sequencing artifacts would be observed for both species, whereas problems associated with one of the three enrichment protocols (two-probe, five-probe and eight-probe enrichment) would be reflected in the corresponding libraries, regardless of the organism considered.
The efficacy of verbal protocol and probe recall SA measurement techniques is noted in terms of observing this effect.
Using think-aloud protocol to probe into these silent instances might possibly yield more insights into the nature of acquiring and processing listening.
The whole-transcriptome amplification protocol and probe design of exon arrays allow a more precise monitoring of such overall gene expression indexes.
This protocol can probe both hyphal and budding Z. tritici morphologies.
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