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The sequencing libraries were prepared using the Illumina genomic DNA sample prep kit according to the protocol, "Preparing Samples for Sequencing Genomic DNA rev B (Illumina)".
Thus, we applied a modified version of the Illumina protocol "Preparing Samples for ChIP Sequencing of DNA" to generate the sequencing library.
The barcode library construction was performed following the protocol "Preparing Long Amplicon Libraries" (Life Technologies) and using the Ion Xpress™ Plus Fragment Library Kit (Life Technologies).
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Simon Pegg has joked that his combat training ahead of Mission: Impossible - Ghost Protocol prepared him for "running down some steps".
A step wise approach for assessment, training and re assessment was carried out as per the protocol prepared by the department of Accident and Emergency Medicine in our multi-specialty hospital.
All authors conceived the idea, prepared the study protocol, prepared the manuscript, and approved the manuscript in its current form.
At the beginning of the process, the methodology was outlined in an evaluation protocol prepared by the Regional Office.
Because of the generally positive results of this pilot [ 6], the countries had a protocol prepared for a main study, named INCANT (INternational CAnnabis Need of Treatment).
DNA material for molecular testing was obtained after bacterial lysis according to the extraction protocol prepared by the Community Reference Laboratory for Antimicrobial Resistance with slight modifications [ 16].
BK and CR led the conceptualization and design of the original project, developed this current protocol, prepared the proposal and obtained funding.
DNA for molecular analysis was obtained after bacterial lysis according to the extraction protocol prepared by the Community Reference Laboratory for Antimicrobial Resistance with slight modifications [ 21].
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