We applied a modified protocol of transformation-associated recombination [28], [29] to isolate individual var genes from P. falciparum strain ITG2F6.
Using a modified protocol for transformation of Halobacterium sp., recombinant plasmids each with one of the SIVsm gene segment, were transformed into the Vac- SD109.
Two protocols of crambe transformation have been reported earlier, one by [ 13] based on hygromycin selection (20 mg · L-1), and another by [ 14] based on kanamycin selection (25 mg · L-1).
Initially, the full collection of yeast mutant strains distributed in seventy-five 96-well microtiter plates (Nunclon) were transformed with the plasmid pJS05 following a protocol of microtiter plate transformation [ 84], using SC-Ura as selection medium.
Lack of transformation protocol is a limitation for functional characterization of genes in Crocus.
Plasmid was linearised with Bln I restriction enzyme and transformation carried out according to the protocol of the Pichia EasyComp™ Transformation kit (Invitrogen).
After transformation using the lithium acetate transformation protocol of Schiestl and Gietz (1989), yeasts were grown with YEPD plus geneticin (200 µg/l) overnight at 28°C to select cells with the KanMX4 integrated module.
Much of the bacterium's unique and fascinating biology and a wide range of transformation protocols have been developed for an impressive number of plant species, varieties, and cultivars.
Tomato is a fleshy and climacteric crop that has several advantages as a fruit development model: economic importance as a crop, small genome, short generation time, availability of transformation protocols and genetic and genomic resources [ 2, 3].
The basic transformation protocol of An [ 65] was used.
